Thought this guide might be useful to all comic collectors as a quick reference to hunting down the First Appearance of their favorite Hero or Villain.  I will update it on a regular basis.  Catch any errors, let me know and I'll correct it!

Absorbing Man - Journey Into Mystery #114 (1965)

Adam Strange - Showcase #17 (1958)

Agatha Harkness - Fantastic Four #94

Alfred - Batman #16 (1943)

Alpha Flight - X-men #120 & X-men #121 (1979)

Amazo - Brave and the Bold #30 (1960)

Angel - X-men #1 (1963)

Animal Man - Strange Adventures #180 (1965)

Antman - Tales to Astonish #27 (1962) #35 (1st in Costume) (1962)

Aquaman - More Fun Comics #73 (1941)

Archangel - cameo X-Factor #23, 1st Full Appearance X-Factor #24

Archie - Pep Comics #22 (1941)

Aries - Thor #129 (1966)

Arion - Warlord #55

Avengers - Avengers #1 (1963)

Banshee - X-men #28 (1967)

Bat Lash - Showcase #76 (1968)

Batgirl - Detective Comics #359 (1967)

Batman - Detective Comics #27 (1939)

Batroc - Tales of Suspense #76

Batwoman - Detective Comics #233 (1956)

Beast - X-men #1 (1963)

Beast (Blue) - Amazing Adventures #11

Betty - Pep Comics #22 (1941)

Beyonder - Secret Wars #1 (1984)

Bizarro - Superboy #68 (1958)

Black Adam (Fawcett/DC) - Marvel Family #1 (1945)

Black Bolt - cameo Fantastic Four #45 1st Full App. Fantastic Four #46 (1965-66)

Black Canary - Flash Comics #86 (1947)

Black Cat - Amazing Spiderman #194 (1979)

Black Knight - Tales to Astonish #52 (1964)

Black Panther - Fantastic Four #52 (1966)

Black Terror - Exciting Comics #9 (1941)

Black Widow - Tales of Suspense #52 (1964)

Blade - Tomb of Dracula #10 (1973)

Blob - X-men #3 (1964)

Blue Beetle - Mystery Men #1 (1940) Silver Age, Blue Beetle #18 (1955) as Ted Kord, Captain Atom #83 (1966)

Boomerang - Tales to Astonish #81 (1966)

Booster Gold - Booster Gold #1 (1986)

Brainiac - Action Comics #242 (1958)

Brotherhood of Evil Mutants - X-men #4 (1964)

Buck Rogers - Famous Funnies #3 (1934)

Buffy the Vampire Slayer - Dark Horse Presents Annual 1998

Bugs Bunny - Looney Tunes #1 (1941)

Bullseye - Nick Fury, Agent of Shield #15 (1969)

Bullseye II - Daredevil #131 (1976)

Cable - as baby Uncanny X-men #201 (1986) 1st full app New Mutants #87 (1990)

Captain America - Captain America Comics #1 (1941) Silver Age app. Avengers #4 (1964)

Captain Atom (Charlton/DC)- Space Adventures #33 (1960)

Captain Boomerang - The Flash #117 (1961)

Captain Marvel (Fawcett/DC) - Whiz Comics #1 (1940)

Captain Marvel Jr. (Fawcett/DC) - Whiz Comics #25 (1941)

Captain Marvel (Marvel) - Marvel Super Heores #12 (1967)

Captain Marvel (Marvel/Female) - Amazing Spiderman Annual #16 (1982)

Captain Triumph - Crack Comics #27 (1943)

Carnage - Amazing Spiderman #344 & 345 (Cameo) #361 (1st Full App.) (1993)

Cat (later becomes Tigra) - The Cat #1 (1972)

Catwoman - as Cat Batman #1 (1940) 1st Full App. as Catwoman in Costume Batman #3 (1940)

Cerebus - Cerebus #1 (1977)

Challengers of the Unknown - Showcase #6 (1957)

Chameleon - Amazing Spiderman #1 (1963)

Champions - The Champions #1 (1975)

Checkmate - Action Comics #598 (1988)

Cloak & Dagger - Spectacular Spiderman #64 (1982)

Colossus - Giant Size X-men #1 (1975)

Conan - Conan the Barbarian #1 (1970)

Concrete - Dark Horse Presents #1 (1986)

Constantine (Hellblazer) - Saga of the Swamp Thing #25 (Cameo) #37 (1st Full App.)

Creeper - Showcase #73 (1968)

Crossbones - Captain America #359 (Cameo) #360 (1st Full App.) (1990)

The Crow (movie) - Caliber Presents #1 (1989)

Cyclops - X-men #1 (1963)

Daffy Duck - Looney Tunes #1 (1941)

Daredevil (Gleason Pub.) - Silver Streak Comics #6 (1940)

Daredevil (Marvel) - Daredevil #1 (1964)

Darkhawk - Darkhawk #1 (1991)

Darkseid - Superman's Pal Jimmy Olsen #134 (1970)

Dawn - Cry for Dawn #1 (1989)

Dazzler - X-men #130 (1980)

Deadman - Strange Adventures #205 (1967)

Deathlok - Astonishing Tales #25 (1974)

Deathstroke - New Teen Titans #2 (1980)

Defenders - Marvel Feature #1 (1971)

Dennis the Menace - Dennis the Menace #1 (1953)

Dick Tracy - Popular Comics #1 (1936)

Doctor Doom - Fantastic Four #5 (1962)

Doctor Fate - More Fun Comics #55 (1940)

Doctor Light - Justice League of America #12 (1962)

Doctor Strange - Strange Tales #110 (1963)

Doc Octopus - Amazing Spiderman #3 (1963)

Doll Man - Feature Comics #27 (1939)

Don Winslow - Popular Comics #1 (1936)

Doomsday - Cameo Superman: The Man of Steel #17 1st Full App. Superman: The Man of Steel #18 (1993)

Electro - Amazing Spiderman #9 (1964)

Elektra - Daredevil #168 (1980)

Elmer Fudd - Looney Tunes #1 (1941)

Elongated Man - The Flash #112 (1960)

Enemy Ace - Our Army at War #151 (1965)

Eternals - Eternals #1 (1976)

Evil Ernie - Evil Ernie #1 (1991)

Fantastic Four - Fantastic Four #1 (1961)

Fighting American - Fighting American #1 (1954)

Fighting Yank - Startling Comics #10 (1941)

Firestorm - Firestorm #1 (1978)

Flame - Wonderworld Comics #3 (1939)

Flaming Carrot - Flaming Carrot Comics #1 (1981)

Flash (DC) - Flash Comics #1 (1940) Silver Age app. Showcase #4 (1956)

Flash Gordon - King Comics #1 (1936)

Flex Mentallo - Cameo Doom Patrol #35 First Full Appearance Doom Patrol #36 (1990)

Frankie Raye - Fantastic Four #164 (1974)

Franklin Richards - Fantastic Four Special #6 (1968)

Galactus - Cameo Fantastic Four #48 1st Full App. Fantastic Four #49 (1966)

Gambit - Cameo X-men Annual 14 (1990) 1st Full App. X-men #266 (1990)

Gen13 - Deathmate: Black (1993)

Ghost Rider - Marvel Spotlight #5 (1972)

Giant Man - previously Ant Man, Tales to Astonish #49 (1963)

Gorgon - Fantastic Four #44 (1965)

Green Arrow - More Fun Comics #73 (1941)

Green Goblin - (Norman Osborn) Amazing Spiderman #14 (1964)

Green Hornet - Green Hornet Comics #1 (1940)

Green Lantern (Original) - All American Comics #16 (1940)

Green Lantern (Hal Jordan) - Showcase #22 (1959)

Green Lantern (Kyle Rayner) - Green Lantern #48 (1994)

Grendel - Primer #2 (1982)

Guardians of the Galaxy - Marvel Super Heroes #18 (1969)

Guy Gardner - Green Lantern #59 (1968)

Gwen Stacy - Amazing Spiderman #31 (1965)

Harry Osborn - Amazing Spiderman #31 (1965)

Havok - w/o Costume X-men #56 (1969) 1st Full app.X-men #58 (1969)

Hawk & Dove - Showcase #75 (1968)

Hawkeye - Tales of Suspense #57 (1964)

Hawkgirl - All Star Comis #5 (1941)

Hawkman - Flash Comics #1 (1940)

He-Man - DC Comics Presents #47 (1982)

Hector Hammond - Green Lantern Vol. 2 #5 (1961)

Hellboy - San Diego Comic Con Comics #2 (1993) in color Next Men #21 (1994)

Hercules - Journey Into Mystery Annual #1 (1965)

High Evolutionary - Thor #134 (1966)

Hobgoblin - Amazing Spiderman #238 (1983)

Howard The Duck - Adventures Into Fear #19

Hulk - Incredible Hulk #1 (1962)

Human Torch - Marvel Comics #1 (1939) Silver Age app. Fantastic Four #1 (1961)

Iceman - X-men #1 (1963)

Impulse - Flash Vol. 2 #92 (1994)

Infinity, Inc. - All Star Squadron #25 (1983)

Inhumans - Fantastic Four #45 (1965)

Invaders - Avengers #71 (1969)

Invincible - Noble Causes: Family Secrets #3 (2002)

Invisible Woman - Fantastic Four #1 (1961)

Ion (Kyle Rayner) - Green Lantern #145

Iron Fist - Marvel Premiere #15 (1974)

Iron Man - Tales of Suspense #39 (1963)

Jigsaw - Amazing Spiderman #162 (1976)

Jimmy Olsen - Action Comics #6 (1938)

John Stewart (Green Lantern) - Green Lantern #87 (1972)

Johnny Quick - More Fun Comics #71 (1941)

Johnny Thunder - Flash Comics #1 (1940)

Jonah Hex - All Star Western #10 (1972)

Joker - Batman #1 (1940)

Jonny Quest - Jonny Quest #1 (1964)

Jubilee - X-men #244 (1989)

Juggernaut - X-men #12 (1965)

Jughead - Pep Comics #22 (1941)

Justice League of America - Brave & the Bold #28 (1960)

Justice Society of America - All Star Comics #3 (1940)

Kamandi, The Last Boy on Earth - Kamandi #1 (1972)

Kandor City - Action Comics #242 (1958)

Kato - Green Hornet Comics #1 (1940)

Kazar - Marvel Comics #1 (1939) Silver Age App. X-men #10 (1964)

Killraven - Amazing Adventures #18 (1973)

Kingpin - Amazing Spiderman #50 (1967)

Kitty Pryde - X-men #129 (1980)

Kraven the Hunter - Amazing Spiderman #15 (1964)

Kull the Conqueror - cameo Conan #1 (1970) 1st Full App. Creatures on the Loose #10 and Kull the Conqueror #1 (1971)

Lady Death - Evil Ernie #1 (1991)

Lady Deathstrike - Alpha Flight #33 (1986)

Leader - Tales to Astonish #62 (1965)

Legion of Super Heroes - Adventure Comics #247 (1958)

Lex Luthor - Action Comics #23 (1940)

Lizard - Amazing Spiderman #6 (1963)

Lobo - Omega Men #3 (1983)

Loki - Journey Into Mystery #85 (1962)

Luke Cage - Hero for Hire, Luke Cage #1 (1972)

Mad Hatter - Detective Comics #230 (1956)

Magneto - X-men #1 (1963)

Man-Bat - Detective Comics #400 (1970)

Man-Thing - Savage Tales #1 (1971)

Martian Manhunter - Detective Comics #225 (1955)

Marvel Girl (Jean Grey) - X-men #1 (1963)

Marvel Zombies - Ultimate Fantastic Four #21

Mary Jane Watson - Amazing Spiderman #25 (1965)

Mask (movie) - Dark Horse Presents #10 (1987)

Mephisto - Silver Surfer Vol. 1 #3 (1968)

Metal Men - Showcase #37 (1962)

Metamorpho - Brave & the Bold #57 (1964)

Mickey Mouse - Mickey Mouse Book No# (1930)

Mimic - X-men #19 (1966)

Miracleman - Miracleman #1 (1985) as Marvelman (Marvelman #25 in 1954)

Mirror Master - The Flash #105 (1959)

Mister Miracle - Mister Miracle #1 (1971)

Mister Sinister - X-men #221 (1989)

Modok - Tales of Suspense #94 (1967)

Molecule Man - Fantastic Four #20 (1963)

Mole Man - Fantastic Four #1 (1961)

Molten Man - Amazing Spiderman #28 (1965)

Moon Knight - Werewolf By Night #32 (1975)

Morbius - Amazing Spiderman #101 (1971)

Mouse Guard - Mouse Guard: The Dark Ghost #1 (2006)

Ms. Marvel (Marvel) - as Carol Danvers Marvel Super Heroes #13 (1967) as hero Ms. Marvel #1 (1977)

Mxyztplk - Superman #30 (1944)

Mysterio - Amazing Spiderman #13 (1964)

Mystery Men (movie) - Flaming Carrot #16 (1987)

Mystique - Ms. Marvel #16 & #17 (Cameo) #18 (1st Full App.) (1978)

New Mutants - Marvel Graphic Novel #4 (1982)

New Warriors - Thor #411 (Cameo) #412 (1st Full App.)

Next Men - Dark Horse Presents #54 (1991)

Nightcrawler - Giant Size X-men #1 (1975)

Nightwing (Dick Grayson) - Tales of the Teen Titans #44 (1984)

Nitro - Captain Marvel #34 (1974)

Nth Man - Marvel Comics Presents #25 (1989)

Nuke - Daredevil #232 (1985)

Odin - Journey Into Mystery #86

O.M.A.C. - O.M.A.C. #1 (1974)

Omega The Unknown - Omega The Unknown #1 (1976)

Orion - The New Gods #1 (1971)

Penguin - Detective Comics #58 (1941)

Phantom - Ace Comics #11 (1938)

Plastic Man - Police Comics #1 (1941)

Pogo the Possum - Animal Comics #1 (1941)

Porky Pig - Looney Tunes #1 (1941)

Power Girl - All Star Comics #58 (1976)

Preacher - Preacher #1 (1995)

Prime - Prime #1 (1993)

Prince Valiant - Ace Comics #26 (1939)

Professor X - X-men #1 (1963)

Puma - Amazing Spiderman #256 (1984)

Punisher (Marvel) - Amazing Spiderman #129 (1974)

Quasar - Captain America #217

Question - Captain Atom #83 (1966)

Quicksilver - X-men #4 (1964)

Ra's al Ghul - Batman #232 (1971)

Ragman - Ragman #1 (1976)

Red Ryder - Crackajack Funnies #9 (1939)

Reed Richards - Fantastic Four #1 (1961)

Red Wolf - Avengers #80 (1970)

Rhino - Amazing Spiderman #41 (1966)

Riddler - Detective Comics #140 (1948) Silver Age App. Batman #171 (1965)

Rip Hunter - Showcase #20 (1959)

Robin I (Dick Grayson) - Detective Comics #38 (1940)

Robin II (Jason Todd) - as Jason Todd Batman #357 (1983) as Robin unofficially Batman #366-367 Officially Batman #368 (1984) "Death" Batman #428 (1988) ressurection Batman Annual #25 (2006)

Robin III (Tim Drake) - as Timothy Drake Batman #436 (1989) as Robin Unofficially Batman #442 Officially Batman #457 (1991)

Rocketeer - Cameo Starslayer #1 1st Full App. Starslayer #2 (1982)

Rogue - Avengers Annual #10 (1981)

Rom - Rom #1 (1979)

Sabretooth - Iron Fist #14 (1977)

Saint of Killers - Preacher #2 (1995)

Sandman - Amazing Spiderman #4 (1963)

Savage Dragon - cameo Megaton #2 1st Full App. Megaton #3 (1985)

Scalphunter - Weird Western Tales #39

Scarecrow - World's Finest Comics #3 (1941)

Scarlet Witch - X-men #4 (1964)

Scorpion - Amazing Spiderman #20 (1964)

Sentinels - X-men #14 (1965)

Sentry - Sentry #1 (2000)

Sgt. Fury - Sgt. Fury & His Howling Commandos #1 (1963)

Sgt. Rock - cameo Our Army at War #81-82 1st Full App. #83 (1959)

Sharon Carter, Agent 13 - As Agent 13, Tales of Suspense #75 As Sharon Carter, Tales of Suspense #76

Sheena, Queen of the Jungle - Jumbo Comics #1 (1938)

She-Hulk - Savage She-Hulk #1 (1980)

Silver Sable - Amazing Spiderman #265 (1985)

Silver Surfer - Fantastic Four #48 (1966)

Sin - Captain America #290

Sin City - Dark Horse Presents 5th Anniversary Special (1991)

Sinestro - Green Lantern #7 (1961)

Son of Satan - cameo Ghost Rider #1 (1973) 1st Full app. Ghost Rider #2 (1973)

Spawn - Spawn #1 (1992)

Spectre - cameo More Fun Comics #51 1st Full App. More Fun Comics #52 (1940)

Speedball - Amazing Spiderman Annual #22 (1988)

Spiderman - Amazing Fantasy #15 (1962)

Spider Girl - What If? Vol.2 #105 (1998)

Spider Woman - Marvel Spotlight #32 (1977)

Spirit - Police Comics #11 (1942)

Squadron Sumpreme - as Squadron Sinister Avengers #70 (1969)

Steel (The Indestructable Man) - Steel #1 (1978)

Storm (Marvel)  - Giant Size X-men #1 (1975)

Sub-Mariner - Marvel Comics #1 (1939)

Suicide Squad - Brave & the Bold #25 (1959)

Sunfire - X-men #64 (1970)

Superboy - More Fun Comics #101 (1945)

Superboy Prime - DC Comics Presents #87 (1985)

Supergirl - Action Comics #252 (1959)

Superman - Action Comics #1 (1938)

Supernova (DC) - World's Finest Comics #178 (1968)

Super Patriot - Nick Fury Agnet of Shield #13 (1969)

Superwoman - DC Comics Presents Annual #2 (1983)

Supreme - Youngblood #3 (1992)

Swamp Thing - House of Secrets #92 (1971)

Talia (Ra's al Ghul's Daughter) - Detective Comics #411 (1971)

Tarzan - Tip Top Comics #1 (1936)

Taskmaster - Avengers #195 (Cameo) #196 (1st Full App.) (1980)

Teen Titans - Brave & the Bold #54 (1964)

Teenage Mutant Ninja Turtles - Ad cameo Gobbledygook #1 1st Full App. Teenage Mutant Ninja Turtles #1 (1984)

Terry & The Pirates - Popular Comics #1 (1936)

Thanos - Iron Man #55 (Cameo) (1973)

Thing - Fantastic Four #1 (1961)

Thor - Journey Into Mystery #83 (1962)

Thunderbird - Giant Size X-Men #1 (1975)

Thunderbolts - Incredible Hulk #449 (1997)

Thunderstrike - Thor #459 (1993)

Tick - The Tick Special Edition #1 (1988)

Tigra (formerly The Cat) - Giant Size Creatures #1 (1974)

Toad - X-men #4 (1964)

Tom & Jerry - Our Gang Comics #1 (1942)

Tomahawk - Star Spangled Comics #69 (1947)

Transformers - Transformers #1 (1984)

Two Face - Detective Comics #66 (1942) Silver Age App. Batman #234 (1971)

Two Gun Kid - Wild West #1 (1948)

Uncle Scrooge McDuck - Four Color #178 (1947)

Underdog - Underdog #1 (1970)

Union Jack I - Invaders #7 (1976)

Union Jack II - Invaders #20 (1977)

Union Jack III - Captain America #254 (1981)

Unknown Soldier - Our Army At War #168 (1968)

U.S. Agent - Captain America #354 (1989)

Usagi Yojimbo - Albedo #2 (1984)

V - V for Vendetta (1988)

Vampirella - Vampirella #1 (1969)

Vault Keeper - War Against Crime #10 (1949)

Venom - Web of Spiderman #18 (behind the scenes) & Amazing Spiderman #298-299 (Cameo) #300 (1st Full Appearance) (1988)

Veronica - Pep Comics #26 (1942)

Vigilante - Action Comics #42 (1941) Silver Age, Justice League of America #78 (1970)

Vixen - Action Comics #521 (1981)

Vulture - Amazing Spiderman #2 (1963)

Warlock - Fantastic Four #66 & 67 (1968)

Warlord - First Issue Special #8 (1975)

War Machine - cameo Iron Man #281 1st Full App. Iron Man #282 (1992)

Warpath - New Mutants #16 (1984)

Wasp - Tales to Astonish #44 (1963)

Watchmen - Watchmen #1 (1986)

Wendigo - Incredible Hulk #162 (1973)

Werewolf By Night - Marvel Spotlight #2 (1972)

White Queen (Emma Frost) - X-men #129 (1980)

Witchblade - Cyblade/Shi: The Battle of the Independents #1

Wolverine - Incredible Hulk #180 (Cameo) #181 (1st Full Apearance) (1974)

Wonder Man - Avengers #9 (1964)

Wonder Woman - All Star Comics #8 (1941)

X - Dark Horse Comics #8 (1993)

X-23 - NYX #3 (2003)

X-Factor - Avengers #263 (1986)

X-Man (Nate Grey) - X-Man #1 (1995)

X-men - X-men #1 (1963)

X-O Manowar - 1st App. of X-O Armor, Magnus #7 (1991-92) Aric X-O Manowar 1st App, X-O Manowar #1 (1992)

Yorick Brown - Y: The Last Man #1 (2002)

Youngblood - Megaton Explosion (1987)

Zatanna - Hawkman #4 (1964)

Zorro - Zorro Four Color #228 (1949)